Application
A. Western blot analysis on 3T3 cell lysates using anti-Phospho-MEKK4 (p80) RabMAb (Cat. #AJ1710a), 1:5000 dilution. Cells were either (A) untreated (B) treated with PDGFApplication
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-Phospho-MKK4 (pX80) RabMAb (Cat. #AJ1710a).| Product Name | SEK (MEKK4) Antibody Phospho (pS80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K4, JNKK1, MKK4, PRKMK4, SERK1, Dual specificity mitogen-activated protein kinase kinase 4, SAPK/ERK kinase 1 |
| Modification | p-S80 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1075Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JNKK1 MEK4 MKK4 PRKMK4 SEK1 SERK1 |
|---|---|
| Alternative Names | MAP2K4 JNKK1 MKK4 PRKMK4 SERK1 Dual specificity mitogen-activated protein kinase kinase 4 SAPK/ERK kinase 1 |
| Molecular Weight(MW) | 44288 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14 |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 6416 |
|---|
Application
A. Western blot analysis on 3T3 cell lysates using anti-Phospho-MEKK4 (p80) RabMAb (Cat. #AJ1710a), 1:5000 dilution. Cells were either (A) untreated (B) treated with PDGF
Application
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-Phospho-MKK4 (pX80) RabMAb (Cat. #AJ1710a).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
