Application
SFN Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SFN antibody detected the SFN protein (arrow).Application
SFN antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of SFN Antibody (C-term) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
SFN Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SFN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HME1 |
|---|---|
| Alternative Names | SFN HME1 14-3-3 protein sigma Epithelial cell marker protein 1 Stratifin |
| Molecular Weight(MW) | 27774 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity) |
| Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium |
| Cellular Localization | Cytoplasm. Nucleus (By similarity). Secreted. Note=May be secreted by a non-classical secretory pathway |
| Entrez Gene | 2810 |
|---|---|
| Nucleotide Accession | NP_006133.1 |
Application
SFN Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SFN antibody detected the SFN protein (arrow).
Application
SFN antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of SFN Antibody (C-term) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
SFN Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
