Application
SIRT6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SIRT6 antibody detected the SIRT6 protein (arrow).Application
SIRT6 Antibody (C-term) (AP14024b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIRT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SIRT6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIR2L6 |
|---|---|
| Alternative Names | SIRT6 SIR2L6 NAD-dependent protein deacetylase sirtuin-6 Regulatory protein SIR2 homolog 6 SIR2-like protein 6 |
| Molecular Weight(MW) | 39119 Da |
| Function | NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate |
| Tissue Specificity | This SIRT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-305 amino acids from the C-terminal region of human SIRT6. |
| Cellular Localization | Nucleus, nucleoplasm. Note=Predominantly nuclear. Associated with telomeric heterochromatin regions |
| Entrez Gene | 51548 |
|---|---|
| Nucleotide Accession | NP_057623.2 NP_001180214.1 |
Application
SIRT6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SIRT6 antibody detected the SIRT6 protein (arrow).
Application
SIRT6 Antibody (C-term) (AP14024b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIRT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
