Application
Western blot analysis of anti-SLBP Pab in Jurkat(left) and T47D (right) cell line lysates (35ug/lane). SLBP (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of SLBP (arrow) using rabbit polyclonal SLBP Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SLBP gene.| Product Name | SLBP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HBP |
|---|---|
| Alternative Names | SLBP HBP Histone RNA hairpin-binding protein Histone stem-loop-binding protein |
| Molecular Weight(MW) | 31286 Da |
| Function | RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Polyribosome- associated. Localizes predominantly in the nucleus at the G1/G2 phases and the beginning of S phase. Through the S phase, partially redistributes to the cytoplasm. Binding to histone mRNA is necessary for cytoplasmic localization. Shuttles between the nucleus and the cytoplasm. Imported in the nucleus by the Importin alpha/Importin beta receptor |
| Entrez Gene | 7884 |
|---|
Application
Western blot analysis of anti-SLBP Pab in Jurkat(left) and T47D (right) cell line lysates (35ug/lane). SLBP (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of SLBP (arrow) using rabbit polyclonal SLBP Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SLBP gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
