Application
Western blot analysis of SLC27A5 Antibody (Center) in Hela cell line lysates (35ug/lane). SLC27A5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human kidney reacted with SLC27A5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SLC27A5 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SLC27A5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC27A5; ACSB; ACSVL6; FACVL3; FATP5; Bile acyl-CoA synthetase; Bile acid-CoA ligase; Cholate--CoA ligase; Fatty acid transport protein 5; Fatty-acid-coenzyme A ligase, very long-chain 3; Solute carrier family 27 member 5; Very long-chain acyl-CoA synthet |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACSB ACSVL6 FACVL3 FATP5 |
|---|---|
| Alternative Names | SLC27A5 ACSB ACSVL6 FACVL3 FATP5 Bile acyl-CoA synthetase Bile acid-CoA ligase Cholate--CoA ligase Fatty acid transport protein 5 Fatty-acid-coenzyme A ligase very long-chain 3 Solute carrier family 27 member 5 Very long-chain acyl-CoA synthet |
| Molecular Weight(MW) | 75385 Da |
| Function | Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol |
| Tissue Specificity | Predominantly expressed in liver. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 10998 |
|---|
Application
Western blot analysis of SLC27A5 Antibody (Center) in Hela cell line lysates (35ug/lane). SLC27A5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human kidney reacted with SLC27A5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SLC27A5 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
