Application
A. Western blot analysis on Jurkat cell lysate using anti-Smad3 RabMAb (Cat. #AJ1724a), dilution 1:5000.Application
B. Immunohistochemical staining of paraffin-embedded human prostate carcinoma using anti-SMAD3 RabMAb (Cat. #AJ1724a).| Product Name | Smad3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD3, MADH3, Mothers against decapentaplegic homolog 3, SMAD 3;hSMAD3;JV15-2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP568Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH3 |
|---|---|
| Alternative Names | SMAD3 MADH3 Mothers against decapentaplegic homolog 3 SMAD 3 hSMAD3 JV15-2 |
| Molecular Weight(MW) | 48081 Da |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator |
| Tissue Specificity | A synthetic peptide corresponding to the internal region of human Smad3 was used as immunogen. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta |
| Entrez Gene | 4088 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Smad3 RabMAb (Cat. #AJ1724a), dilution 1:5000.
Application
B. Immunohistochemical staining of paraffin-embedded human prostate carcinoma using anti-SMAD3 RabMAb (Cat. #AJ1724a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM221453