Application
SMC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SMC3 antibody detected the SMC3 protein (arrow).| Product Name | SMC3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMC3; BAM; BMH; CSPG6; SMC3L1; Structural maintenance of chromosomes protein 3; Basement membrane-associated chondroitin proteoglycan; Chondroitin sulfate proteoglycan 6; Chromosome-associated polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAM BMH CSPG6 SMC3L1 |
|---|---|
| Alternative Names | SMC3 BAM BMH CSPG6 SMC3L1 Structural maintenance of chromosomes protein 3 Basement membrane-associated chondroitin proteoglycan Chondroitin sulfate proteoglycan 6 Chromosome-associated polypeptide |
| Molecular Weight(MW) | 141542 Da |
| Function | Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement |
| Tissue Specificity | This SMC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-431 amino acids from the Central region of human SMC3. |
| Cellular Localization | Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser- 1083 is preferentially associated with unsynapsed chromosomal regions (By similarity) |
| Entrez Gene | 9126 |
|---|---|
| Nucleotide Accession | NP_005436.1 |
Application
SMC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SMC3 antibody detected the SMC3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
