Application
Western blot analysis of SYMD2 (arrow) using rabbit polyclonal SYMD2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2) (Origene Technologies).Application
SMYD2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).Application
SMYD2 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).| Product Name | SMYD2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMYD2; KMT3C; N-lysine methyltransferase SMYD2; HSKM-B; Histone methyltransferase SMYD2; Lysine N-methyltransferase 3C; SET and MYND domain-containing protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT3C |
|---|---|
| Alternative Names | SMYD2 KMT3C N-lysine methyltransferase SMYD2 HSKM-B Histone methyltransferase SMYD2 Lysine N-methyltransferase 3C SET and MYND domain-containing protein 2 |
| Molecular Weight(MW) | 49688 Da |
| Function | Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB |
| Tissue Specificity | This SMYD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129~158 amino acids from the N-term of human SYMD2. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Nucleus (By similarity) |
| Entrez Gene | 56950 |
|---|
Application
Western blot analysis of SYMD2 (arrow) using rabbit polyclonal SYMD2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2) (Origene Technologies).
Application
SMYD2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).
Application
SMYD2 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
