Application
Western blot analysis of SNAIL (arrow) using rabbit polyclonal hSNAIL-D24 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAIL antibody (N-term D24) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SNAIL Antibody (N-term D24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SNAH |
|---|---|
| Alternative Names | SNAI1 SNAH Zinc finger protein SNAI1 Protein snail homolog 1 |
| Molecular Weight(MW) | 29083 Da |
| Function | Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself |
| Tissue Specificity | Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines |
| Cellular Localization | Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs |
| Entrez Gene | 6615 |
|---|
Application
Western blot analysis of SNAIL (arrow) using rabbit polyclonal hSNAIL-D24 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAIL antibody (N-term D24) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
