Application
Western blot analysis of anti-SPAK Pab in mouse liver tissue lysate. SPAK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
SPAK Antibody (A363) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the SPAK antibody detected the SPAK protein (arrow).Application
Western blot analysis of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.| Product Name | SPAK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STK39; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; DCHT; Serine/threonine-protein kinase 39 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPAK |
|---|---|
| Alternative Names | STK39 SPAK STE20/SPS1-related proline-alanine-rich protein kinase DCHT Serine/threonine-protein kinase 39 |
| Molecular Weight(MW) | 59474 Da |
| Function | May act as a mediator of stress-activated signals. |
| Tissue Specificity | Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis |
| Cellular Localization | Cytoplasm (Probable). Nucleus (Probable). Note=Nucleus when caspase-cleaved (Probable) |
| Entrez Gene | 27347 |
|---|
Application
Western blot analysis of anti-SPAK Pab in mouse liver tissue lysate. SPAK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
SPAK Antibody (A363) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the SPAK antibody detected the SPAK protein (arrow).
Application
Western blot analysis of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
