Application
Western blot analysis of SPP1 (arrow) using rabbit polyclonal SPP1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPP1 gene (Lane 2).Application
SPP1 Antibody (N-term) (RB18956) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SPP1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPP1; BNSP; OPN; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BNSP OPN |
|---|---|
| Alternative Names | SPP1 BNSP OPN Osteopontin Bone sialoprotein 1 Nephropontin Secreted phosphoprotein 1 Urinary stone protein Uropontin |
| Molecular Weight(MW) | 35423 Da |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction |
| Tissue Specificity | Bone. Found in plasma. |
| Cellular Localization | Secreted. |
| Entrez Gene | 6696 |
|---|
Application
Western blot analysis of SPP1 (arrow) using rabbit polyclonal SPP1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPP1 gene (Lane 2).
Application
SPP1 Antibody (N-term) (RB18956) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SPP1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
