Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Stat3 (pY705) RabMAb (Cat. #AJ1739d), 1:20,000 dilution. Cells were either (A) untreated (B) treated with IFN-alpha.Application
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Phospho STAT3 (pY705) RabMAb (Cat. #AJ1739d).Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho STAT3 (pY705) RabMAb (Cat. #AJ1739d)| Product Name | Stat-3 Antibody Phospho (pY705) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT3, APRF, Signal transducer and activator of transcription 3, Acute-phase response factor |
| Modification | p-Y705 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2147Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:10000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APRF |
|---|---|
| Alternative Names | STAT3 APRF Signal transducer and activator of transcription 3 Acute-phase response factor |
| Molecular Weight(MW) | 88068 Da |
| Function | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA |
| Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1 |
| Entrez Gene | 6774 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Stat3 (pY705) RabMAb (Cat. #AJ1739d), 1:20,000 dilution. Cells were either (A) untreated (B) treated with IFN-alpha.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Phospho STAT3 (pY705) RabMAb (Cat. #AJ1739d).
Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho STAT3 (pY705) RabMAb (Cat. #AJ1739d)| Application Notes | WB:1:10000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
