Application
Western blot analysis of STRAP Antibody (Center) in Y79 cell line lysates (35ug/lane). STRAP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with STRAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using STRAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of STRAP (arrow) using rabbit polyclonal STRAP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STRAP gene.| Product Name | STRAP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STRAP; MAWD; UNRIP; Serine-threonine kinase receptor-associated protein; MAP activator with WD repeats; UNR-interacting protein; WD-40 repeat protein PT-WD |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAWD UNRIP |
|---|---|
| Alternative Names | STRAP MAWD UNRIP Serine-threonine kinase receptor-associated protein MAP activator with WD repeats UNR-interacting protein WD-40 repeat protein PT-WD |
| Molecular Weight(MW) | 38438 Da |
| Function | The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein |
| Tissue Specificity | This STRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~143 amino acids from the Center region of human STRAP. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Localized predominantly in the cytoplasm but also found in the nucleus |
| Entrez Gene | 11171 |
|---|
Application
Western blot analysis of STRAP Antibody (Center) in Y79 cell line lysates (35ug/lane). STRAP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with STRAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using STRAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of STRAP (arrow) using rabbit polyclonal STRAP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STRAP gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
