Application
A. Western blot analysis of Anti-SUMO-1 RabMAb (Cat. #AJ1746a), dilution 1:1,000. A-Hela B-NIH 3T3Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-SUMO-1 RabMAb (Cat. #AJ1746a).Application
C. Immunofluorescent staining of HeLa cells using anti-SUMO-1 RabMAb (Cat. #AJ1746a).| Product Name | SUMO-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO1, SMT3C, SMT3H3, UBL1, Small ubiquitin-related modifier 1, Sentrin;Ubiquitin-like protein SMT3C;SMT3 homolog 3;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein UBL1;GAP-modifying protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y299 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3C SMT3H3 UBL1 |
|---|---|
| Alternative Names | SUMO1 SMT3C SMT3H3 UBL1 Small ubiquitin-related modifier 1 Sentrin Ubiquitin-like protein SMT3C SMT3 homolog 3 Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 |
| Molecular Weight(MW) | 11557 Da |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2 Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-term of human SUMO-1 was used as immunogen. |
| Cellular Localization | Nucleus membrane. Nucleus speckle. Cytoplasm. Note=Recruited by BCL11A into the nuclear body (By similarity) |
| Entrez Gene | 7341 |
|---|
Application
A. Western blot analysis of Anti-SUMO-1 RabMAb (Cat. #AJ1746a), dilution 1:1,000. A-Hela B-NIH 3T3
Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-SUMO-1 RabMAb (Cat. #AJ1746a).
Application
C. Immunofluorescent staining of HeLa cells using anti-SUMO-1 RabMAb (Cat. #AJ1746a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
