Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
The SUMO2 C-term Antibody is used in Western blot to detect SUMO2 in GST-SUMO2 fusion protein (lane 1) and HL60 cell lysate (lane 2).Application
Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.| Product Name | SUMO2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO2; SMT3A; SMT3H2; Small ubiquitin-related modifier 2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3A SMT3H2 |
|---|---|
| Alternative Names | SUMO2 SMT3A SMT3H2 Small ubiquitin-related modifier 2 HSMT3 SMT3 homolog 2 SUMO-3 Sentrin-2 Ubiquitin-like protein SMT3A |
| Molecular Weight(MW) | 10871 Da |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins |
| Tissue Specificity | Broadly expressed. |
| Cellular Localization | Nucleus. Note=Nuclear bodies. |
| Entrez Gene | 6613 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
The SUMO2 C-term Antibody is used in Western blot to detect SUMO2 in GST-SUMO2 fusion protein (lane 1) and HL60 cell lysate (lane 2).
Application
Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
