Application
A. Western blot analysis on (A) HeLa and (B) Molt-4 cell lysates using anti-Survivin RabMAb (Cat. #AJ1747b), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Survivin RabMAb (Cat. #AJ1747b).| Product Name | Survivin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BIRC5, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor survivin;Apoptosis inhibitor 4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2675 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | API4 IAP4 |
|---|---|
| Alternative Names | BIRC5 API4 IAP4 Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 |
| Molecular Weight(MW) | 16389 Da |
| Function | Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform |
| Tissue Specificity | Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export |
| Entrez Gene | 332 |
|---|
Application
A. Western blot analysis on (A) HeLa and (B) Molt-4 cell lysates using anti-Survivin RabMAb (Cat. #AJ1747b), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Survivin RabMAb (Cat. #AJ1747b).| Application Notes | WB:1:5000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
