Application
SUV39H1 western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the SUV39H1 antibody detected the SUV39H1 protein (arrow).| Product Name | SUV39H1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUV39H1; KMT1A; SUV39H; Histone-lysine N-methyltransferase SUV39H1; Histone H3-K9 methyltransferase 1; Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT1A SUV39H |
|---|---|
| Alternative Names | SUV39H1 KMT1A SUV39H Histone-lysine N-methyltransferase SUV39H1 Histone H3-K9 methyltransferase 1 Lysine N-methyltransferase 1A Position-effect variegation 3-9 homolog Suppressor of variegation 3-9 homolog 1 |
| Molecular Weight(MW) | 47907 Da |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone- modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus |
| Tissue Specificity | Purified recombinant GST fusion protein encoding N-terminal of human SUV39H1. |
| Cellular Localization | Nucleus. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin |
Application
SUV39H1 western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the SUV39H1 antibody detected the SUV39H1 protein (arrow).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
