Application
Western blot analysis of anti-hSUV39H2-K315 Pab (Cat. AP1281b) in 293 and HL60 cell line lysates (35ug/lane). hSUV39H2-K315(arrow) was detected using the purified Pab (1:62 dilution).| Product Name | SUV39H2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUV39H2; KMT1B; Histone-lysine N-methyltransferase SUV39H2; Histone H3-K9 methyltransferase 2; Lysine N-methyltransferase 1B; Suppressor of variegation 3-9 homolog 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT1B |
|---|---|
| Alternative Names | SUV39H2 KMT1B Histone-lysine N-methyltransferase SUV39H2 Histone H3-K9 methyltransferase 2 Lysine N-methyltransferase 1B Suppressor of variegation 3-9 homolog 2 |
| Molecular Weight(MW) | 46682 Da |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis |
| Tissue Specificity | This SUV39H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308~337 amino acids from the C-terminal of human SUV39H2. |
| Cellular Localization | Nucleus (By similarity). Chromosome, centromere (By similarity). Note=Associates with centromeric constitutive heterochromatin (By similarity) |
Application
Western blot analysis of anti-hSUV39H2-K315 Pab (Cat. AP1281b) in 293 and HL60 cell line lysates (35ug/lane). hSUV39H2-K315(arrow) was detected using the purified Pab (1:62 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
