Application
TADA3L Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).Application
Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term)(Cat#AP14766b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.| Product Name | TADA3L Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TADA3; ADA3; TADA3L; Transcriptional adapter 3; ADA3 homolog; STAF54; Transcriptional adapter 3-like |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADA3 TADA3L |
|---|---|
| Alternative Names | TADA3 ADA3 TADA3L Transcriptional adapter 3 ADA3 homolog STAF54 Transcriptional adapter 3-like |
| Molecular Weight(MW) | 48902 Da |
| Function | Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 10474 |
|---|---|
| Nucleotide Accession | NP_006345.1 NP_597814.1 |
Application
TADA3L Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).
Application
Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term)(Cat#AP14766b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
