Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THEM2 antibody (C-term) (Cat.#1460b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of THEM2 Antibody (C-term) polyclonal antibody in mouse liver tissue lysates (35ug/lane). THEM2 (arrow) was detected using the purified Pab (1:60 dilution).| Product Name | THEM2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | THEM2 |
|---|---|
| Alternative Names | ACOT13 THEM2 Acyl-coenzyme A thioesterase 13 Thioesterase superfamily member 2 |
| Molecular Weight(MW) | 14960 Da |
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro) |
| Tissue Specificity | This THEM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82~112 amino acids from the C-terminal region of human THEM2. |
| Cellular Localization | Cytoplasm (By similarity). Mitochondrion (By similarity) |
| Entrez Gene | 55856 |
|---|
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THEM2 antibody (C-term) (Cat.#1460b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of THEM2 Antibody (C-term) polyclonal antibody in mouse liver tissue lysates (35ug/lane). THEM2 (arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
