Application
TNFRSF11B Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TNFRSF11B antibody detected the TNFRSF11B protein (arrow).Application
Confocal immunofluorescent analysis of TNFRSF11B Antibody (Center) (Cat#AP13738c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
TNFRSF11B Antibody (Center) (AP13738c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNFRSF11B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TNFRSF11B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFRSF11B; OCIF; OPG; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCIF OPG |
|---|---|
| Alternative Names | TNFRSF11B OCIF OPG Tumor necrosis factor receptor superfamily member 11B Osteoclastogenesis inhibitory factor Osteoprotegerin |
| Molecular Weight(MW) | 46026 Da |
| Function | Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis |
| Tissue Specificity | Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung |
| Cellular Localization | Secreted. |
| Entrez Gene | 4982 |
|---|---|
| Nucleotide Accession | NP_002537.3 |
Application
TNFRSF11B Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TNFRSF11B antibody detected the TNFRSF11B protein (arrow).
Application
Confocal immunofluorescent analysis of TNFRSF11B Antibody (Center) (Cat#AP13738c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
TNFRSF11B Antibody (Center) (AP13738c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNFRSF11B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
