Application
Western blot analysis of TOP2A Antibody (C-term) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (Cat#AP9248b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | TOP2A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TOP2 |
|---|---|
| Alternative Names | TOP2A TOP2 DNA topoisomerase 2-alpha DNA topoisomerase II alpha isozyme |
| Molecular Weight(MW) | 174385 Da |
| Function | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes |
| Tissue Specificity | This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1174~1204 amino acids from the C-terminal region of human TOP2A. |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm |
| Entrez Gene | 7153 |
|---|
Application
Western blot analysis of TOP2A Antibody (C-term) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (Cat#AP9248b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
