Application
A. Western blot analysis using anti- Topo II alpha Rabbit Monoclonal on HeLa. (Cat. #AJ1780a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Topo II alpha RabMAb(Cat. #AJ1780a).| Product Name | Topoisomerase-II Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1102Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TOP2 |
|---|---|
| Alternative Names | TOP2A TOP2 DNA topoisomerase 2-alpha DNA topoisomerase II alpha isozyme |
| Molecular Weight(MW) | 174385 Da |
| Function | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes |
| Tissue Specificity | A synthetic peptide corresponding to C-terminal residues of human Topo IIα was used as an immunogen. |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm |
| Entrez Gene | 7153 |
|---|
Application
A. Western blot analysis using anti- Topo II alpha Rabbit Monoclonal on HeLa. (Cat. #AJ1780a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Topo II alpha RabMAb(Cat. #AJ1780a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
