Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b), 1:1000 dilution. Cells were either (A) treated (B) untreated with AP.Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b).| Product Name | Topoisomerase-II Antibody (alpha) Phospho-pT1343 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme |
| Modification | p-T1343 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1054Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TOP2 |
|---|---|
| Alternative Names | TOP2A TOP2 DNA topoisomerase 2-alpha DNA topoisomerase II alpha isozyme |
| Molecular Weight(MW) | 174385 Da |
| Function | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343. |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm |
| Entrez Gene | 7153 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b), 1:1000 dilution. Cells were either (A) treated (B) untreated with AP.
Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
