Application
TRIM25 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRIM25 antibody detected the TRIM25 protein (arrow).| Product Name | TRIM25 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EFP RNF147 ZNF147 |
|---|---|
| Alternative Names | TRIM25 EFP RNF147 ZNF147 E3 ubiquitin/ISG15 ligase TRIM25 Estrogen-responsive finger protein RING finger protein 147 Tripartite motif-containing protein 25 Ubiquitin/ISG15-conjugating enzyme TRIM25 Zinc finger protein 147 |
| Molecular Weight(MW) | 70973 Da |
| Function | Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies |
| Entrez Gene | 7706 |
|---|---|
| Nucleotide Accession | NP_005073.2 |
Application
TRIM25 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRIM25 antibody detected the TRIM25 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
