Application
TRIM32 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TRIM32 antibody detected the TRIM32 protein (arrow).Application
TRIM32 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM32 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TRIM32 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRIM32 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM32; HT2A; E3 ubiquitin-protein ligase TRIM32; 72 kDa Tat-interacting protein; Tripartite motif-containing protein 32; Zinc finger protein HT2A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HT2A |
|---|---|
| Alternative Names | TRIM32 HT2A E3 ubiquitin-protein ligase TRIM32 72 kDa Tat-interacting protein Tripartite motif-containing protein 32 Zinc finger protein HT2A |
| Molecular Weight(MW) | 71989 Da |
| Function | Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo |
| Tissue Specificity | Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle |
| Cellular Localization | Cytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus |
| Entrez Gene | 22954 |
|---|---|
| Nucleotide Accession | NP_001093149.1 NP_036342.2 |
Application
TRIM32 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TRIM32 antibody detected the TRIM32 protein (arrow).
Application
TRIM32 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM32 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TRIM32 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
