Application
TRIM9 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM9 antibody detected the TRIM9 protein (arrow).Application
TRIM9 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRIM9 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM9; KIAA0282; RNF91; E3 ubiquitin-protein ligase TRIM9; RING finger protein 91; Tripartite motif-containing protein 9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0282 RNF91 |
|---|---|
| Alternative Names | TRIM9 KIAA0282 RNF91 E3 ubiquitin-protein ligase TRIM9 RING finger protein 91 Tripartite motif-containing protein 9 |
| Molecular Weight(MW) | 79177 Da |
| Function | E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation |
| Tissue Specificity | Brain. Highly expressed in the cerebral cortex (at protein level). Severely decreased in the affected brain areas in Parkinson disease and dementia with Lewy bodies |
| Cellular Localization | Cytoplasm. Cell projection, dendrite. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle (By similarity). Cell junction, synapse (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Enriched at synaptic terminals where it exists in a soluble form and a synaptic vesicle- associated form. Associated with the cytoskeleton (By similarity) Found in proximal dendrites of pyramidal neurons in the cerebral cortex and hippocampus, and Purkinje cells in the cerebellum |
| Entrez Gene | 114088 |
|---|---|
| Nucleotide Accession | NP_443210.1 NP_055978.4 |
Application
TRIM9 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM9 antibody detected the TRIM9 protein (arrow).
Application
TRIM9 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
