Application
A. Western blot analysis on Jurkat cell lysate using anti-TSC2 (C-term) RabMAb (Cat. #AJ1798a), dilution 1:1,000Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-TSC2 RabMAb (C-term) (Cat. #AJ1798a).| Product Name | TSC2/Tuberin Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC2, TSC4, Tuberin, Tuberous sclerosis 2 protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y320 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TSC4 |
|---|---|
| Alternative Names | TSC2 TSC4 Tuberin Tuberous sclerosis 2 protein |
| Molecular Weight(MW) | 200608 Da |
| Function | In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors |
| Tissue Specificity | Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes |
| Entrez Gene | 7249 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-TSC2 (C-term) RabMAb (Cat. #AJ1798a), dilution 1:1,000
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-TSC2 RabMAb (C-term) (Cat. #AJ1798a).| Application Notes | WB:1:200~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM223756