Application
A. Western blot analysis on (A) U937, (B) HeLa, (C) Jurkat and (D) HUVEC cell lysates using anti-UBC9 RabMAb (Cat. #AJ1805a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-UBC9 RabMAb (Cat. #AJ1805a).| Product Name | UBC-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2I, UBC9, UBCE9, SUMO-conjugating enzyme UBC9, SUMO-protein ligase;Ubiquitin-conjugating enzyme E2 I;Ubiquitin-protein ligase I;Ubiquitin carrier protein I;Ubiquitin carrier protein 9;p18 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2938Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBC9 UBCE9 |
|---|---|
| Alternative Names | UBE2I UBC9 UBCE9 SUMO-conjugating enzyme UBC9 SUMO-protein ligase Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I Ubiquitin carrier protein I Ubiquitin carrier protein 9 p18 |
| Molecular Weight(MW) | 18007 Da |
| Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5 Essential for nuclear architecture and chromosome segregation |
| Tissue Specificity | Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus |
| Cellular Localization | Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity) |
| Entrez Gene | 7329 |
|---|
Application
A. Western blot analysis on (A) U937, (B) HeLa, (C) Jurkat and (D) HUVEC cell lysates using anti-UBC9 RabMAb (Cat. #AJ1805a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-UBC9 RabMAb (Cat. #AJ1805a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
