Application
Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml)| Product Name | UBE2C Antibody (N-term G25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2C; UBCH10; Ubiquitin-conjugating enzyme E2 C; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBCH10 |
|---|---|
| Alternative Names | UBE2C UBCH10 Ubiquitin-conjugating enzyme E2 C UbcH10 Ubiquitin carrier protein C Ubiquitin-protein ligase C |
| Molecular Weight(MW) | 19652 Da |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit |
| Tissue Specificity | This UBE2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human UBE2C. |
| Entrez Gene | 11065 |
|---|
Application
Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml)| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
