Application
UBE2N Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBE2N antibody detected the UBE2N protein (arrow).Application
UBE2N Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2N Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | UBE2N Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2N; BLU; Ubiquitin-conjugating enzyme E2 N; Bendless-like ubiquitin-conjugating enzyme; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BLU |
|---|---|
| Alternative Names | UBE2N BLU Ubiquitin-conjugating enzyme E2 N Bendless-like ubiquitin-conjugating enzyme Ubc13 UbcH13 Ubiquitin carrier protein N Ubiquitin-protein ligase N |
| Molecular Weight(MW) | 17138 Da |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity) |
| Tissue Specificity | This UBE2N antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human UBE2N. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 7334 |
|---|---|
| Nucleotide Accession | NP_003339.1 |
Application
UBE2N Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBE2N antibody detected the UBE2N protein (arrow).
Application
UBE2N Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2N Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
