Application
The anti-UBE3A Pab is used in Western blot to detect UBE3A in HeLa cell lysate.Application
UBE3A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE3A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UBE3A Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UBE3A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE3A; E6AP; EPVE6AP; HPVE6A; Ubiquitin-protein ligase E3A; E6AP ubiquitin-protein ligase; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | E6AP EPVE6AP HPVE6A |
|---|---|
| Alternative Names | UBE3A E6AP EPVE6AP HPVE6A Ubiquitin-protein ligase E3A E6AP ubiquitin-protein ligase Human papillomavirus E6-associated protein Oncogenic protein-associated protein E6-AP Renal carcinoma antigen NY-REN-54 |
| Molecular Weight(MW) | 100688 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo |
| Tissue Specificity | This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175~205 amino acids from the N-terminal region of human UBE3A. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 7337 |
|---|
Application
The anti-UBE3A Pab is used in Western blot to detect UBE3A in HeLa cell lysate.
Application
UBE3A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE3A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UBE3A Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM224114