Application
UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).Application
Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term)(Cat#AP13949a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
UGT1A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | UGT1A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UGT1A1; GNT1; UGT1; UDP-glucuronosyltransferase 1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GNT1 UGT1 |
|---|---|
| Alternative Names | UGT1A1 GNT1 UGT1 UDP-glucuronosyltransferase 1-1 Bilirubin-specific UDPGT isozyme 1 UDP-glucuronosyltransferase 1-A UDP-glucuronosyltransferase 1A1 |
| Molecular Weight(MW) | 59591 Da |
| Function | UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone |
| Tissue Specificity | Expressed in liver. Not expressed in skin or kidney |
| Cellular Localization | Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 54658 |
|---|---|
| Nucleotide Accession | NP_000454.1 |
Application
UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).
Application
Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term)(Cat#AP13949a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
UGT1A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM224202