Application
Western blot analysis of ULK2 Antibody (C-term) (Cat.# AP8105b) in mouse brain tissue lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.| Product Name | ULK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ULK2; KIAA0623; Serine/threonine-protein kinase ULK2; Unc-51-like kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0623 |
|---|---|
| Alternative Names | ULK2 KIAA0623 Serine/threonine-protein kinase ULK2 Unc-51-like kinase 2 |
| Molecular Weight(MW) | 112694 Da |
| Function | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons |
| Tissue Specificity | This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 620~649 amino acids from the C-terminal sequence of human ULK2. |
| Cellular Localization | Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane |
| Entrez Gene | 9706 |
|---|
Application
Western blot analysis of ULK2 Antibody (C-term) (Cat.# AP8105b) in mouse brain tissue lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
