Application
Western blot analysis of UNG Antibody (C-term) in T47D cell line lysates (35ug/lane). UNG (arrow) was detected using the purified Pab.Application
UNG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UNG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UNG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of UNG Antibody (C-term)(Cat#AP9936b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | UNG Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UNG; DGU; UNG1; UNG15; Uracil-DNA glycosylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DGU UNG1 UNG15 |
|---|---|
| Alternative Names | UNG DGU UNG1 UNG15 Uracil-DNA glycosylase |
| Molecular Weight(MW) | 34645 Da |
| Function | Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine |
| Tissue Specificity | Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells |
| Cellular Localization | Isoform 1: Mitochondrion. |
| Entrez Gene | 7374 |
|---|
Application
Western blot analysis of UNG Antibody (C-term) in T47D cell line lysates (35ug/lane). UNG (arrow) was detected using the purified Pab.
Application
UNG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UNG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UNG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of UNG Antibody (C-term)(Cat#AP9936b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
