Application
Western blot analysis of UNC5C Antibody (Center) in K562 cell line lysates (35ug/lane). UNC5C (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with UNC5C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using UNC5C Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UNC5C Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UNC5C; UNC5H3; Netrin receptor UNC5C; Protein unc-5 homolog 3; Protein unc-5 homolog C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UNC5H3 |
|---|---|
| Alternative Names | UNC5C UNC5H3 Netrin receptor UNC5C Protein unc-5 homolog 3 Protein unc-5 homolog C |
| Molecular Weight(MW) | 103146 Da |
| Function | Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (By similarity) |
| Tissue Specificity | Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung |
| Cellular Localization | Membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 8633 |
|---|
Application
Western blot analysis of UNC5C Antibody (Center) in K562 cell line lysates (35ug/lane). UNC5C (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with UNC5C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using UNC5C Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
