Application
The anti-USP12 Pab is used in Western blot to detect USP12 in mouse kidney tissue lysate.Application
Western blot analysis of USP12 Antibody (N-term) in CEM cell line lysates (35ug/lane). USP12 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | USP12 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP12; UBH1; USP12L1; Ubiquitin carboxyl-terminal hydrolase 12; Deubiquitinating enzyme 12; Ubiquitin thioesterase 12; Ubiquitin-hydrolyzing enzyme 1; Ubiquitin-specific-processing protease 12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBH1 USP12L1 |
|---|---|
| Alternative Names | USP12 UBH1 USP12L1 Ubiquitin carboxyl-terminal hydrolase 12 Deubiquitinating enzyme 12 Ubiquitin thioesterase 12 Ubiquitin-hydrolyzing enzyme 1 Ubiquitin-specific-processing protease 12 |
| Molecular Weight(MW) | 42858 Da |
| Function | Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2 |
| Tissue Specificity | This USP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human USP12. |
Application
The anti-USP12 Pab is used in Western blot to detect USP12 in mouse kidney tissue lysate.
Application
Western blot analysis of USP12 Antibody (N-term) in CEM cell line lysates (35ug/lane). USP12 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
