Application
USP13 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the USP13 antibody detected the USP13 protein (arrow).Application
USP13 Antibody (N-term) (AP14518a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | USP13 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP13; ISOT3; Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Isopeptidase T-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ISOT3 |
|---|---|
| Alternative Names | USP13 ISOT3 Ubiquitin carboxyl-terminal hydrolase 13 Deubiquitinating enzyme 13 Isopeptidase T-3 Ubiquitin thioesterase 13 Ubiquitin-specific-processing protease 13 |
| Molecular Weight(MW) | 97327 Da |
| Function | Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13 Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data |
| Tissue Specificity | Highly expressed in ovary and testes. |
| Entrez Gene | 8975 |
|---|---|
| Nucleotide Accession | NP_003931.2 |
Application
USP13 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the USP13 antibody detected the USP13 protein (arrow).
Application
USP13 Antibody (N-term) (AP14518a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
