Application
Western blot analysis of USP2 Antibody (N-term) in HL60 cell line lysates (35ug/lane). USP2 (arrow) was detected using the purified Pab.Application
IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer.Application
Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (T22) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.| Product Name | USP2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBP41 |
|---|---|
| Alternative Names | USP2 UBP41 Ubiquitin carboxyl-terminal hydrolase 2 41 kDa ubiquitin-specific protease Deubiquitinating enzyme 2 Ubiquitin thioesterase 2 Ubiquitin-specific-processing protease 2 |
| Molecular Weight(MW) | 68072 Da |
| Function | Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells |
| Tissue Specificity | Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level) |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, perinuclear region (By similarity). Note=Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane (By similarity) |
| Entrez Gene | 9099 |
|---|
Application
Western blot analysis of USP2 Antibody (N-term) in HL60 cell line lysates (35ug/lane). USP2 (arrow) was detected using the purified Pab.
Application
IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer.
Application
Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (T22) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
