Application
The anti-USP5 Pab is used in Western blot to detect USP5 in HL-60 cell lysate.| Product Name | USP5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ISOT |
|---|---|
| Alternative Names | USP5 ISOT Ubiquitin carboxyl-terminal hydrolase 5 Deubiquitinating enzyme 5 Isopeptidase T Ubiquitin thioesterase 5 Ubiquitin-specific-processing protease 5 |
| Molecular Weight(MW) | 95786 Da |
| Function | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition |
| Tissue Specificity | This USP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 491~520 amino acids from the C-terminal region of human USP5. |
| Entrez Gene | 8078 |
|---|
Application
The anti-USP5 Pab is used in Western blot to detect USP5 in HL-60 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
