Application
USP8-C1072 western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the USP8 antibody detected the USP8 protein (arrow).| Product Name | USP8 (UBPY) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0055, UBPY |
|---|---|
| Alternative Names | USP8 KIAA0055 UBPY Ubiquitin carboxyl-terminal hydrolase 8 Deubiquitinating enzyme 8 Ubiquitin isopeptidase Y Ubiquitin thioesterase 8 Ubiquitin-specific-processing protease 8 |
| Molecular Weight(MW) | 127523 Da |
| Function | Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation Required to enter into S phase in response to serum stimulation May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1 |
| Tissue Specificity | This USP8 (UBPY) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1065~1094 amino acids from the C-terminal region of human USP8. |
| Cellular Localization | Cytoplasm. Nucleus (By similarity). Endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein |
| Entrez Gene | 9101 |
|---|
Application
USP8-C1072 western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the USP8 antibody detected the USP8 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
