Application
A. Western blot analysis on 5 ng of recombinant protein using anti-VEGF RabMAb (Cat. #AJ1813a), dilution 1:1000.Application
B. Immunohistochemical staining of hemangioma using anti-VEGF RabMAb (Cat. #AJ1813a).Application
C. Immunofluorescent staining of HUVEC cells using anti-VEGF RabMAb (Cat. #AJ1813a).| Product Name | VEGF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1176Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VEGF |
|---|---|
| Alternative Names | VEGFA VEGF Vascular endothelial growth factor A Vascular permeability factor |
| Molecular Weight(MW) | 27042 Da |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth |
| Tissue Specificity | Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed |
| Cellular Localization | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin |
| Entrez Gene | 7422 |
|---|
Application
A. Western blot analysis on 5 ng of recombinant protein using anti-VEGF RabMAb (Cat. #AJ1813a), dilution 1:1000.
Application
B. Immunohistochemical staining of hemangioma using anti-VEGF RabMAb (Cat. #AJ1813a).
Application
C. Immunofluorescent staining of HUVEC cells using anti-VEGF RabMAb (Cat. #AJ1813a).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
