Application
WWTR1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WWTR1 antibody detected the WWTR1 protein (arrow).| Product Name | WWTR1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WWTR1; TAZ; WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TAZ |
|---|---|
| Alternative Names | WWTR1 TAZ WW domain-containing transcription regulator protein 1 Transcriptional coactivator with PDZ-binding motif |
| Molecular Weight(MW) | 44101 Da |
| Function | Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition |
| Tissue Specificity | Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue |
| Cellular Localization | Nucleus. Cytoplasm. Note=Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15 |
| Entrez Gene | 25937 |
|---|---|
| Nucleotide Accession | NP_001161752.1 NP_056287.1 |
Application
WWTR1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WWTR1 antibody detected the WWTR1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
