Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-YAP65 (pS127) RabMAb (Cat. #AJ1822b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with FBS + calyculin A.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-YAP65 RabMAb (Cat. #AJ1822b).| Product Name | YAP65 Antibody Phospho (pS127) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YAP1, YAP65, 65 kDa Yes-associated protein, YAP65 |
| Modification | p-S127 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1675Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | YAP65 |
|---|---|
| Alternative Names | YAP1 YAP65 65 kDa Yes-associated protein YAP65 |
| Molecular Weight(MW) | 54462 Da |
| Function | Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3) |
| Tissue Specificity | Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus. Note=Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. PTPN14 induces translocation from the nucleus to the cytoplasm |
| Entrez Gene | 10413 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-YAP65 (pS127) RabMAb (Cat. #AJ1822b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with FBS + calyculin A.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-YAP65 RabMAb (Cat. #AJ1822b).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
