Application
A. Western blot analysis on Jurkat cell lysate using anti-Phospho-ZAP70 (pY292) RabMAb (Cat. #AJ1824c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadateApplication
B. Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate -treated (red) using anti- Cbl RabMAb (Cat. #AJ1824c), and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292).| Product Name | ZAP70 Antibody Phospho (pY292) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y292 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1073 |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SRK |
|---|---|
| Alternative Names | ZAP70 SRK Tyrosine-protein kinase ZAP-70 70 kDa zeta-associated protein Syk-related tyrosine kinase |
| Molecular Weight(MW) | 69872 Da |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2 Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) |
| Tissue Specificity | Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) |
| Entrez Gene | 7535 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Phospho-ZAP70 (pY292) RabMAb (Cat. #AJ1824c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate
Application
B. Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate -treated (red) using anti- Cbl RabMAb (Cat. #AJ1824c), and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292).| Application Notes | WB:1:500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
