Application
Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35ug/lane). ARAF1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | A-RAF Antibody (ARAF1) (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARAF1 PKS PKS2 |
|---|---|
| Alternative Names | ARAF ARAF1 PKS PKS2 Serine/threonine-protein kinase A-Raf Proto-oncogene A-Raf Proto-oncogene A-Raf-1 Proto-oncogene Pks |
| Molecular Weight(MW) | 67585 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus |
| Tissue Specificity | Predominantly in urogenital tissues. |
| Entrez Gene | 369 |
|---|
Application
Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35ug/lane). ARAF1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
