Application
A2M Antibody (N-term) western blot analysis in mouse plasma lysates (35ug/lane).This demonstrates the A2M antibody detected the A2M protein (arrow).| Product Name | A2M Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A2M; CPAMD5; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPAMD5 |
|---|---|
| Alternative Names | A2M CPAMD5 Alpha-2-macroglobulin C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
| Molecular Weight(MW) | 163291 Da |
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase |
| Tissue Specificity | Secreted in plasma. |
| Cellular Localization | Secreted. |
| Entrez Gene | 2 |
|---|---|
| Nucleotide Accession | NP_000005.2 |
Application
A2M Antibody (N-term) western blot analysis in mouse plasma lysates (35ug/lane).This demonstrates the A2M antibody detected the A2M protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
