Application
ACF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).Application
ACF Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ACF Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A1CF; ACF; ASP; APOBEC1 complementation factor; APOBEC1-stimulating protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACF ASP |
|---|---|
| Alternative Names | A1CF ACF ASP APOBEC1 complementation factor APOBEC1-stimulating protein |
| Molecular Weight(MW) | 65202 Da |
| Function | Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay |
| Tissue Specificity | Widely expressed with highest levels in brain, liver, pancreas, colon and spleen |
| Cellular Localization | Nucleus. Endoplasmic reticulum (By similarity). Cytoplasm. Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity) Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1 |
Application
ACF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).
Application
ACF Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ACF Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
