Application
Western blot analysis of ACTC antibody (Center) in K562 cell line lysates (35ug/lane). ACTC (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle with ACTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using ACTC Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACTC Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTA2; ACTSA; ACTVS; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTSA ACTVS |
|---|---|
| Alternative Names | ACTA2 ACTSA ACTVS Actin aortic smooth muscle Alpha-actin-2 Cell growth-inhibiting gene 46 protein |
| Molecular Weight(MW) | 42009 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197~227 amino acids from the Center region of human ACTC. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 59 |
|---|
Application
Western blot analysis of ACTC antibody (Center) in K562 cell line lysates (35ug/lane). ACTC (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle with ACTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using ACTC Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
