Application
Western blot analysis of ACVR1B (arrow) using rabbit polyclonal ACVR1B Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACVR1B gene.Application
ACVR1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACVR1B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ACVR1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACVR1B; ACVRLK4; ALK4; Activin receptor type-1B; Activin receptor type IB; Activin receptor-like kinase 4; Serine/threonine-protein kinase receptor R2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACVRLK4 ALK4 |
|---|---|
| Alternative Names | ACVR1B ACVRLK4 ALK4 Activin receptor type-1B Activin receptor type IB Activin receptor-like kinase 4 Serine/threonine-protein kinase receptor R2 |
| Molecular Weight(MW) | 56807 Da |
| Function | Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2 |
| Tissue Specificity | Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 91 |
|---|---|
| Nucleotide Accession | NP_004293.1 NP_064733.3 |
Application
Western blot analysis of ACVR1B (arrow) using rabbit polyclonal ACVR1B Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACVR1B gene.
Application
ACVR1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACVR1B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM225835